Titlebook: Investigation of Membrane-Located Receptors; Eric Reid,G. M. W. Cook,D. J. Morré Book 1984 Springer Science+Business Media New York 1984 G

不近人情

Molecular Genetics Applied to Receptor Analysisits subunits present and intact, we can predict that the cDNA approach will become, instead, the route for determining the amino acid sequences of receptor subunits in general. Cloning of receptor genes will introduce, furthermore, many other new possibilities into receptor analysis, as I shall briefly consider here.

Infant

Epithelial Sodium Channels: Ligand Binding Techniques in Intact Tissues and Homogenatesels, while those in kidney may be related to non-electrogenic sodium-proton exchange. Emphasis is placed throughout on physiological manipulations which affect both function and binding, and on the wide discrepancies in the amount of binding found in intact and homogenized tissues.

Preamble

GUILT

Hormone Receptor — Adenylate Cyclase Interaction: Superpotency, Superaffinity and Desensitization Phinity. (3) Catecholamine desensitization as studied with turkey erythrocytes is due to a regulatory defect at the level of nucleotide regulatory proteins (N. -protein J. In general this article concerns some interactions of the hormone receptor-N-protein -AC complex.

Palpate

amina-testing saga surpassing any past experience. Amongst the numerous authors - a notably eminent bevy - some were angelic and others suffered harassment to produce, amidst day-to-day pressures, an eventual article in the cause of receptor investigation; few took exception to the strong editing th

Gentry

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Strategy in Receptor Binding Studies. This article considers some of the methods for optimization of the binding assay technique, for visualization and analysis of binding data, and for investigation of the complexities of the binding of ligands to receptors.

disparage

Principles of Receptor Binding Assays: The Gaba Receptorg of the fundamental principles that govern this procedure. Some important considerations include choice of ligand, isotopic species, tissue preparation and incubation conditions. Thus, the manner in which tissue is stored and prepared, as well as the incubation buffer, temperature, time and method

FEIGN

Specificity Criteria for Receptor Sites in Binding Studiesficient. There is no receptor without physiological response. Criteria are discussed with a number of examples, positive and negative. Also advocated is the postulate of receptor homogeneity, such that receptor sites fora given neurotransmitter are identical in all parts of the body: any odd results

Horizon

Target Size Analysis of Cell Surface Receptors and Adenylate Cyclaseor solubilization. Known doses of high-energy radiation, viz. electrons from a linear accelerator, are delivered homogeneously to a sample of a crude enzyme or receptor preparation. To avoid indirect effects of radiation the sample has to be either dry (lyophilized) or kept frozen at a constant temp