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Titlebook: Investigation of Membrane-Located Receptors; Eric Reid,G. M. W. Cook,D. J. Morré Book 1984 Springer Science+Business Media New York 1984 G

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Isolation and Characterization of Spiny- (Clathrin)-Coated Membranes and Vesicles from Rodent Liver or-mediated endocytosis and secretion. Clathrin is the major coat protein of both endocytotic and exocytotic vesicles and membranes in this and other tissues, and presumably plays some yet unknown role in membrane translocations.
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https://doi.org/10.1007/978-1-4684-4631-9Glycin; Monoclonal Antibodies; Nucleotide; chemistry; cytotoxicity; glycoprotein; liposomes; preparation; pr
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Where Do We Stand on Receptors Today?This Introduction represents remarks on the current scene in relation to the diverse presentations on methodology that follow.
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Receptor Purification: A Brief OverviewThis terse survey, written by request, complements A. Levitzki’s scene-setting survey (#A-1), and does not warrant a summary. The General Index entry ‘Purification approaches’ may usefully be consulted also.
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978-1-4684-4633-3Springer Science+Business Media New York 1984
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Analyzing the Steps in Coupling the Opiate Receptor to Adenylate Cyclase in Neuroblastoma × Glioma Hlation of GTP hydrolysis; and inhibition of cAMP formation. Aspects considered include the use of sulph-hydryl- alkylatin g and oxidizing agents in elucidating the nature of the ternary complex and its role in GTP hydrolysis and cAMP synthesis blockage. The release step and, finally, the overall process of opiate inhibition of AC are considered.
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Architecture of Plasma Membrane Hormone Receptor-Effector Systemses involved are discussed briefly along with the methodology implicit in such studies. Techniques utilized in these studies may allow differential elution of components of the receptor-effector system.
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Determination of Molecular Weights of Receptors in Solution and ,. This is best used in a relative mode, since attempts to use it absolutely with respect to the radiation dose applied have been susceptible to considerable errors. Applica-tions of this technique which give useful information on receptors and their multiple binding sites in the membrane are also reviewed.
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