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Titlebook: Invertebrate Tissue Culture Methods; Jun Mitsuhashi Book 2002 Springer-Verlag Tokyo 2002 Annelida.Chordata.Echinodermata.Insecta.Mollusca.

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Basic Information and OverviewIt is not necessary to spray alcohol in the cabinet before use. Turn the UV light off and turn the fan and fluorescent light on. The gas burner cannot be lit before the fan is operational in most laminar flow cabinets. While working, keep the front glass shutter as low as possible, leaving only enough space to insert your arms under the shutter.
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Arthropods Other Than InsectsArachnida is the second most studied arthropoda for cell culture. Several continuously growing cell lines have been established from tick embryos.
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ProchordataCirculating hemocytes of the solitary protochordates do not survive for prolonged periods in vitro. Subsequently, pharyngeal tissue, which is considered a hemocytopoietic tissue, has been cultured by Raftos et al. (1990). The following procedure is based on their method.
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AnnelidaOocytes cultures have been made using the medium of Heacox et al., modified Cecil’s medium, or modified TC-199 medium (see Appendix 1). The following is the method of Heacox et al. (1983) to cultivate oocytes of the polychaete ..
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