Titlebook: Invertebrate Tissue Culture Methods; Jun Mitsuhashi Book 2002 Springer-Verlag Tokyo 2002 Annelida.Chordata.Echinodermata.Insecta.Mollusca.

DALLY

向外

小故事

Book 2002ow­ ing cells from pupal ovaries of the emperor gum moth, Antheraea euca­ lypti. At that time, I was trying to obtain growing cells from leafhoppers. Grace‘s method could not be applied directly to my culture because of the differences in species, the size of the insects, and the tissue to be cul­ t

agonist

旧病复发

结果

抱狗不敢前

Facilities and Equipmentrvation and maintenance, has become common practice (Fig. 2). Washing and sterilization may be performed in a regular laboratory, because modern sterilizing equipment does not liberate much water vapor and heat. However, if strict sterility is required, it may be better to place a laminar flow cabinet in a sterile room (Fig. 3).

COM

Insecta (Diptera)rious species of mosquitoes. The aim of establishing cell lines from mosquitoes is mostly to study arboviruses in vector cell culture with the hope of producing interferons. Other than . and mosquitoes, very few dipteran insects have been cultured.

Watemelon

Facilities and Equipmentusing sterile techniques. If possible, three separate rooms, i.e., a sterile room, an observation and maintenance room, and a washing and sterilizing room, are desirable (Fig. 1). However, this requires a large space. After the development of laminar flow cabinets (hoods), most investigators now use

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