Titlebook: Intestinal Differentiated Cells; Methods and Protocol Paloma Ordóñez-Morán Book 2023 The Editor(s) (if applicable) and The Author(s), under

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Visualization of Differentiated Cells in 3D and 2D Intestinal Organoid Culturesrphogenetic protein (BMP), epidermal growth factor (EGF), and Notch signaling pathways. Based on this understanding, a combination of stem cell niche factors, EGF, Noggin, and the Wnt agonist R-spondin was shown to enable the growth of mouse intestinal stem cells and the formation of organoids with

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Fluorescence Intensity and , (FLIM) of Cell Differentiation in the Small Intestinal Organoids Using nctional cellular status in response to intrinsic and extrinsic (e.g., in the presence of microbiota) factors. While the use of transgenic animal models expressing biosensor fluorescent proteins can be laborious and not compatible with clinical samples and patient-derived organoids, the use of fluor

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In Vitro Morphogenesis and Differentiation of Human Intestinal Epithelium in a Gut-on-a-Chipcture and function of the human intestine. Here, we describe an experimental protocol to build an organomimetic gut-on-a-chip microdevice that allows inducing 3D morphogenesis of human intestinal epithelium using Caco-2 cells or intestinal organoid cells. Under physiological flow and physical motion

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Book 2023stinal differentiated cell, RNAseq, single-cell RNAseq transcriptional profiling methods, isolation of intestinal crypts, and methods for studying intestinal differentiated cancer cells. Written in the format of the highly successful .Methods in Molecular Biology .series, each chapter includes an in

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1064-3745 ation advice from the experts.This volume details the most up-to-date methods of intestinal differentiation program. Chapters guide readers through techniques to characterize intestinal differentiated cell, RNAseq, single-cell RNAseq transcriptional profiling methods, isolation of intestinal crypts,

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Novel Approach to Measure Transepithelial Electrical Resistance in Intestinal Cellsing treated with RA, and any changes in response to RA were monitored after treatment. The ECIS recorded changes in impedance in response to the treatment and vehicle. This methodology poses as a novel way to record the behavior of colonic cells and opens new avenues for in vitro research.

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In Vitro Morphogenesis and Differentiation of Human Intestinal Epithelium in a Gut-on-a-Chips, intestinal epithelium spontaneously recreates 3D epithelial morphology in a gut-on-a-chip that offers enhanced mucus production, epithelial barrier, and longitudinal host-microbe co-culture. This protocol may provide implementable strategies to advance traditional in vitro static cultures, human microbiome studies, and pharmacological testing.