Titlebook: Intestinal Differentiated Cells; Methods and Protocol Paloma Ordóñez-Morán Book 2023 The Editor(s) (if applicable) and The Author(s), under

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In Vivo Model for Isolating Epithelial Cells of the Anorectal Transition Zoneial region called transition zone (TZ). TZ are small areas found in numerous places in the body such as between the esophagus and the stomach, in the cervix, in the eye, and between the anal canal and the rectum. These zones are associated with diverse pathologies such as cancers; however, the cellu

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Epigenetic and Transcriptional Dynamics of Notch Program in Intestinal Differentiationve for maintaining intestinal homeostasis. In the hierarchical model, intestinal differentiation is defined by the stepwise acquisition of lineage-specific mature cell features, where Notch signaling and lateral inhibition instructively regulate the cell-fate decisions. Recent studies reveal a broad

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Directed Differentiation of Murine and Human Small Intestinal Organoids Toward All Mature Lineageselial biology and can be used to study homeostatic turnover of the corresponding tissue. Organoids can be enriched for the various mature lineages which allows studies of the respective differentiation processes and of the diverse cellular functions. Here we describe mechanisms of intestinal fate sp

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Modeling Notch Activity and Lineage Decisions Using Intestinal Organoidst fate decision for ISC and early progenitors to make is between secretory (Paneth cell, goblet cell, enteroendocrine cell, or tuft cell) and absorptive (enterocyte and M cell) lineages. Using genetic and pharmacological approaches, in vivo studies in the past decade have revealed that Notch signali

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Generation of Fetal Intestinal Organoids and Their Maturation into Adult Intestinal Cellsthelium into the mature intestinal tract composed of the columnar epithelium and characterized by the unique crypt-villus structures. In mice, maturation of fetal gut precursor cells into adult intestinal cells starts around embryonic day (E) 16.5, during which adult intestinal stem cells and their