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Titlebook: Interleukin Protocols; Luke A. J. O’Neill,Andrew Bowie Book 2001 Humana Press 2001

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Measurement of Interleukin-2 by Bioassaych as cellular proliferation, chemotaxis, or cytotoxicity. These assays often use established cell lines that depend for their continued growth and survival on the presence of particular cytokine(s). The detection of the cytokine may be made directly using the cell line of choice or via a second cel
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Assaying Interleukin-15f three subunits: IL-2 receptor β-chain, the y-chain (βc) shared by IL-15, IL-2, IL-4, IL-7, and IL-9 receptors, and a unique a-chain. The α-chain of the IL-15 receptor (IL-15Rα) and IL-2Rα share a fragmentary sequence homology and structural similarities, and both proteins contain a conserved “sush
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Detection of Cytokines by Immunohistochemistry tumor necrosis factors (TNF-α and -β3), interferons (IFN-α, -β, and -γ), transforming growth factor families (TGF-α and -β), activin, inhibin, chemotactic factors, and epidermal, fibroblast, insulin-like, nerve, and platelet-derived growth factors.
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Book 2001 belong to a broader family termed cytokines. Collectively these proteins are the key orchestrators of host defense and the response to tissue injury. There are currently 23 different interleukins (numbered from IL-1 to IL-23), although the full extent of the interleukin family will only become clea
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Quantification of Mouse IL-6 and TNF- α mRNA Using Xplore Assaysplore® (Endogen, Woburn, MA) assay for the quantification of cytokine mRNA. These microtiter plate-based assays are rapid (under 6 h), quantitative over three orders of magnitude, and have no risk of falsepositive values from contamination.
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