Titlebook: Influenza Virus; Methods and Protocol Yohei Yamauchi Book 2018 Springer Science+Business Media, LLC, part of Springer Nature 2018 Vaccine d

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Deciphering Virus Entry with Fluorescently Labeled Viral Particles, the procedure to fluorescently label viral particles. We also illustrate how to use this powerful tool to decipher the entry of viruses with the most recent fluorescence-based techniques such as high-speed confocal and total internal reflection microscopy, flow cytometry, and fluorimetry.

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3D Electron Microscopy (EM) and Correlative Light Electron Microscopy (CLEM) Methods to Study Virusntegrated, known as correlative light electron microscopy (CLEM), are also explained in this chapter. All of these methods are applicable to any kind of cultured cells, including influenza virus-infected cells.

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Influenza Virus-Liposome Fusion Studies Using Fluorescence Dequenching and Cryo-electron Tomographytive time scales of liposomal content leakage or transfer vs. lipid merging. In addition, cryo-ET offers a means of imaging three-dimensional snapshots of different stages of virus-liposome fusion such as prefusion, fusion intermediates, and postfusion.

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Infection of Cultured Mammalian Cells with Aerosolized Influenza Virus,iquid inocula. We have recently shown that cultured cells can become successfully infected after exposure to aerosolized influenza virus. In this chapter, we describe the methodology employed, including the operation of aerosolization instrumentation and calculation of infectious dose, both in experimental planning and after exposure occurs.

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Yohei YamauchiIncludes cutting-edge methods and protocols for the study of the influenza virus.Provides step-by-step detail essential for reproducible results.Contains key notes and implementation advice from the e

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