Titlebook: Influenza Virus; Methods and Protocol Yohei Yamauchi Book 2018 Springer Science+Business Media, LLC, part of Springer Nature 2018 Vaccine d

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Assays to Measure the Activity of Influenza Virus Polymerase,A virus RdRp consists of a heterotrimeric complex of the proteins PB1, PB2, and PA. The RdRp is associated with the incoming influenza A viral RNA (vRNA) genome bound by the viral nucleoprotein (NP), in complexes called viral ribonucleoproteins, vRNPs. During the viral replication cycle, the RdRp sn

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Book 2018tic investigation of virus-host interactions. Chapters detail a broad range of methods from diagnosis, virus propagation, proteomics, haploid screening, lentiviral screening, virus entry, single molecule RNA imaging, correlative light and electron microscopy (CLEM), EM, light-sheet microscopy, bioch

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Purification and Proteomics of Influenza Virions,tor binding and cleaving activity. Material purified in this way can be used for a variety of downstream applications, including proteomics. As a detailed example of this, the chapter also describes a standard workflow for analyzing the protein composition of concentrated virions by liquid chromatography and tandem mass spectrometry.

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Haploid Screening for the Identification of Host Factors in Virus Infection,an intracellular receptor sparked the development of tailored strategies to interfere with viral infection. The “single tube” pooled screening technique presented here does not require any automation or robotics and is potentially applicable to any virus able to infect HAP1 cells.

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Correlative Light and Electron Microscopy of Influenza Virus Entry and Budding,py of virus-infected human lung carcinoma A549 cells with high-pressure freezing (HPF) and in-resin fluorescence CLEM and the Tokuyasu CLEM method. This approach forms a basis to study the virus life cycle and virus-host interactions at the ultrastructural level.

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