Titlebook: Influenza Virus; Methods and Protocol Yohei Yamauchi Book 2018 Springer Science+Business Media, LLC, part of Springer Nature 2018 Vaccine d

Inveterate

相互影响

Phenotypic Lentivirus Screens to Identify Antiviral Single Domain Antibodies,, or depletion. This approach has been remarkably successful, as, for example, demonstrated by the independent identification of the endosomal membrane protein Niemann-Pick C1 as an essential factor for Ebola virus infection in both small compound and insertional mutagenesis screens (Côté, Nature 47

nephritis

Deciphering Virus Entry with Fluorescently Labeled Viral Particles, cell surface. Then a complex series of events, highly dynamic, tightly intricate, and often hard to investigate, follows. This includes virus displacement at the plasma membrane, binding to receptors, signaling, internalization, and release of the viral genome and material into the cytosol. In the

Harridan

PRO

芭蕾舞女演员

3D Electron Microscopy (EM) and Correlative Light Electron Microscopy (CLEM) Methods to Study Virusviral particles and their propagation to new hosts. Understanding how viruses interact with their hosts requires the use of high-resolution techniques for the direct visualization of these interactions. Here electron microscopy (EM) methods are described that allow the 3D ultrastructural analysis of

LINE

Correlative Light and Electron Microscopy of Influenza Virus Entry and Budding,hment of hemagglutinin to cell surface sialic acids, followed by endocytic uptake, vesicular transport along microtubules, low-pH-mediated viral membrane fusion with the late endosomal membrane, capsid uncoating, viral ribonucleoprotein (vRNP) release, and nuclear import of vRNPs. Here we show a bas

Diaphragm

stressors

Metal-Tagging Transmission Electron Microscopy and Immunogold Labeling on Tokuyasu Cryosections to ultrastructural level, by integrating biochemistry, molecular biology, and morphology, have allowed to study the functions of viral macromolecular complexes within the cellular context. Here, we describe a strategy for imaging influenza virus ribonucleoproteins in infected cells with two complementa

malign

Live Imaging of Influenza Viral Ribonucleoproteins Using Light-Sheet Microscopy, make up its genome and subsequent coordinated assembly as they egress from the host cell. Fast, time-resolved volumetric live cell imaging offers a powerful tool for understanding the various host mechanisms hijacked by the virus. Here, we describe the methods necessary for generating influenza vir