Titlebook: Inclusion Bodies; Methods and Protocol Julian Kopp,Oliver Spadiut Book 2023 The Editor(s) (if applicable) and The Author(s), under exclusiv

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Unit Operation-Spanning Investigation of the Redox Systemes are formed during the refolding step. Here, a protocol to identify suitable redox conditions for solubilization and refolding is presented. For this purpose, a multivariate approach spanning the unit operations solubilization and refolding is used.

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Bidirectional Promoter Libraries Enable the Balanced Co-expression of Two Target Genes in levated enzyme activity with one of the .designed promoters of the library. The new toolbox offers a straightforward one-pot cloning approach and is highly modular. As such, the method presented here should be of great interest to any gene co-expression study.

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Development of Solubilization and Refolding Buffersizing and refolding buffers) for each individual protein should be experimentally obtained. Here, we present a simple protocol for development of solubilizing and refolding buffers for successful recovery of pure bioactive proteins from IBs.

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Using High Pressure and Alkaline pH for Refoldingt proteins in . using a rich medium to obtain high expression levels, bacterial lysis, and washing of the IB to obtain products of high purity, and, finally, the solubilization and high yield of refolded proteins using high pressure and alkaline pH.

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The Purification of Heme Peroxidases from , Inclusion Bodies: A Success Story Shown by the Example ocommercially available plant-derived HRP. Moreover, we believe that this procedure can also be used to process other peroxidases of family II and III of the plant peroxidase superfamily, as they all share the same relevant features like disulfide bonds and a heme group.