书目名称 | Inclusion Bodies |
副标题 | Methods and Protocol |
编辑 | Julian Kopp,Oliver Spadiut |
视频video | |
概述 | Includes cutting-edge techniques.Provides step-by-step detail essential for reproducible results.Contains key implementation advice from the experts |
丛书名称 | Methods in Molecular Biology |
图书封面 |  |
描述 | This detailed volume presents a series of protocols dealing with different aspects of inclusion body (IB) processing, from cloning procedures to purification of refolded product. Commencing with chapters on upstream processing, looking into different expression strategies for IB production, the book continues with downstream applications, highlighting early protein purification and subsequent analytics, as well as success stories of IB-based processes. Written for the highly successful .Methods in Molecular Biology. series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. .Authoritative and practical, .Inclusion Bodies: Methods and Protocols. serves as an ideal resource for facilitating diverse aspects of IB processing.. |
出版日期 | Book 2023 |
关键词 | Recombinant proteins; Biopharmaceuticals; IB processing; Biosimilars; Escherichia coli; Fed-batch cultiva |
版次 | 1 |
doi | https://doi.org/10.1007/978-1-0716-2930-7 |
isbn_softcover | 978-1-0716-2932-1 |
isbn_ebook | 978-1-0716-2930-7Series ISSN 1064-3745 Series E-ISSN 1940-6029 |
issn_series | 1064-3745 |
copyright | The Editor(s) (if applicable) and The Author(s), under exclusive license to Springer Science+Busines |