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Titlebook: In Situ Hybridization Protocols; K. H. Andy Choo Book Oct 19941st edition Humana Press 1995

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Gene Mapping Using 3H-Labeled Heterologous Probesng may be accomplished without the necessity of cloning each gene. Thus it is possible to construct physical genetic maps for any mammalian species without the time-consuming process of cloning the homologous sequences.
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Generation of Alphoid DNA Probes for Fluorescence In Situ Hybridization (FISH) Using the Polymerase s. Blocks of consecutive monomers comprise a chromosome-specific higher-order repeat of up to several kilobases (kb) in size. For most human chromosomes, specific multimeric higher-order repeat units have been described (for review, . ref. .).
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Primed In Situ (PRINS) Labeling of DNAetection methods have become available, resulting in a much more widespread use of . hybridization. These methods have become of great value, e.g., as a supplement to traditional cytogenetic analysis in the identification and quantification of numerical and/or structural aberrations in both clinical genetics and cancer diagnosis.
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Free Chromatin Mapping by FISHn provide all the information required for detailed analysis of genomes with greater than several billion base pairs (bp) and each method has its limitation in range of resolution. A combination of different techniques is therefore necessary for any large-scale gene mapping endeavors.
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Book Oct 19941st editionThere is very little doubt that the applications of this te- nique will multiply in coming years since older methods are rapidly superseded by newer ones that enable the investigation of even more sophisticated biological questions and clinical problems. For the n- ice, there should thus be a sense
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putes the idea that spontaneous spoken discourse is flawed, .In contrast to traditional approaches of mainstream psycholinguists, the authors of Communicating with One Another approach spontaneous spoken discourse as a dynamic process, rich with structures, patterns, and rules other than conventiona
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