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Titlebook: In Situ Hybridization Protocols; K. H. Andy Choo Book Oct 19941st edition Humana Press 1995

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书目名称In Situ Hybridization Protocols
编辑K. H. Andy Choo
视频video
丛书名称Methods in Molecular Biology
图书封面Titlebook: In Situ Hybridization Protocols;  K. H. Andy Choo Book Oct 19941st edition Humana Press 1995
描述In situ hybridization is now recognized as an important technique in many areas of molecular biological research and its associated cli- cal studies. There is very little doubt that the applications of this te- nique will multiply in coming years since older methods are rapidly superseded by newer ones that enable the investigation of even more sophisticated biological questions and clinical problems. For the n- ice, there should thus be a sense of urgency to acquire and extend the range of this technique in order not to miss out on the large body of information that can result from its application. During the explosive growth phase of most technical innovations, such knowledge is dif- cult to acquire systematically. The aim of In Situ Hybridization Pro- cols then is to bring together a wide range of detailed laboratory protocols covering different areas of the in situ hybridization technique in order to assist such workers in rapidly advancing toward this goal. With this aim in mind, each of the protocol chapters opens with a detailed Materials section, and an easy-to-follow Methods section. Many readers will find the section headed Notes particularly useful. This s- tion contains
出版日期Book Oct 19941st edition
版次1
doihttps://doi.org/10.1385/0896032809
issn_series 1064-3745
copyrightHumana Press 1995
The information of publication is updating

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Mapping Human YAC Clones by Fluorescence In Situ Hybridization Using Alu-PCR from Single Yeast ColonThe yeast artificial chromosome (YAC) cloning system is capable of cloning large segments of DNA (50–2000 kb) from complex genomes (.). YAC genomic libraries have been constructed using DNA from human as well as other species and have been proven to be powerful tools for the analysis of the human genome.
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Reverse Chromosome Paintinglabeled, the composition of the aberrant chromosome is revealed by the distribution of the fluorescence signals obtained with the different probes. The combination of different labeled probes and multicolor techniques simplifies the evaluation.
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In Situ Hybridization Using Synthetic Oligomers as Probes for Centromere and Telomere Repeats the genome have the same terminal repeat. During the last 5 years, . hybridization of the telomere sequence has been used for a variety of studies (.– .). The integrity of the telomere as a structural and functional necessity for accurate chromosomal distribution during cell division has been supported by these studies.
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