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Titlebook: Immunophenotyping; Methods and Protocol J. Philip McCoy, Jr Book 2019 Springer Science+Business Media, LLC, part of Springer Nature 2019 fl

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Book 2019ook cover topics such as constructions of high dimensions fluorescence and mass cytometry panels; fluorescence barcoding; using dried or lyophilized reagents; and immunophenotypic examples of specific cell types. The book concludes with a discussion on the critical roles of quality control and immun
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Guidelines for Gating Flow Cytometry Data for Immunological Assays,the display of example data, collected by academic, government, and industry representatives. These guidelines should be of value to both novice and experienced flow cytometrists analyzing a wide variety of immunological assays.
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Immunophenotyping of Human Innate Lymphoid Cells,ILCs in healthy and pathologic conditions to streamline a uniform immunophenotyping. Standardized ILC monitoring techniques will accelerate our understanding of these effector innate immune cells and ultimately facilitate their targeting in the context of infection, cancer, autoimmune disease, and transplantation.
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Rare Event Phenotyping and Molecular Characterization: Circulating Tumor Cells,for rare cells of interest. Multiparametric flow-cytometric panels allow probing of numerous extracellular markers for immunophenotyping, while whole transcriptome analysis contributes to molecular characterization of individual CTCs. Index sorting links single CTC proteogenomics information.
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Immunophenotyping by Mass Cytometry,mples and can measure 40–50 immunophenotypic markers at a time. This chapter describes most of the commonly used methods for performing basic immunophenotyping experiments by mass cytometry, and how this can be combined with measurement of cellular functional properties.
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Immunophenotyping of Acute Myeloid Leukemia, useful in the initial diagnosis but also in monitoring and determining prognosis of the disease through minimal residual disease (MRD) testing. This chapter provides an overview of procedures for specimen processing, staining, and immunophenotyping of AML and describes the principles of data analysis for AML classification and MRD testing.
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