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Titlebook: Immunophenotyping; Methods and Protocol J. Philip McCoy, Jr Book 2019 Springer Science+Business Media, LLC, part of Springer Nature 2019 fl

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发表于 2025-3-21 18:35:33 | 显示全部楼层 |阅读模式
书目名称Immunophenotyping
副标题Methods and Protocol
编辑J. Philip McCoy, Jr
视频video
概述Includes cutting-edge methods and protocols.Provides step-by-step detail essential for reproducible results.Contains key notes and implementation advice from the experts
丛书名称Methods in Molecular Biology
图书封面Titlebook: Immunophenotyping; Methods and Protocol J. Philip McCoy, Jr Book 2019 Springer Science+Business Media, LLC, part of Springer Nature 2019 fl
描述.This volume presents the latest collection of immunophenotypic techniques and applications used in research and clinical settings. Chapters in this book cover topics such as constructions of high dimensions fluorescence and mass cytometry panels; fluorescence barcoding; using dried or lyophilized reagents; and immunophenotypic examples of specific cell types. The book concludes with a discussion on the critical roles of quality control and immunophenotyping in the clinical environment. Written in the highly successful .Methods in Molecular Biology. series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls..Cutting-edge and comprehensive, .Immunophenotyping: Methods and Protocols. is a valuable resource for any researchers, clinician, or scientist interested in learning more about this evolvingfield. .
出版日期Book 2019
关键词fluorescence-based cytometers; lyophilized reagents; mass cytometry; B lymphocytes; intracellular cytoki
版次1
doihttps://doi.org/10.1007/978-1-4939-9650-6
isbn_softcover978-1-4939-9652-0
isbn_ebook978-1-4939-9650-6Series ISSN 1064-3745 Series E-ISSN 1940-6029
issn_series 1064-3745
copyrightSpringer Science+Business Media, LLC, part of Springer Nature 2019
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978-1-4939-9652-0Springer Science+Business Media, LLC, part of Springer Nature 2019
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Enumeration of Plasmacytoid Dendritic Cells in Peripheral Blood and Bone Marrow by Flow-Cytometric in normal condition and diseases. They activate T cells and play a critical role in immune tolerance. In this chapter we review the immunophenotypic features of pDCs and provide a practical protocol for pDCs enumeration in the peripheral blood and bone marrow samples.
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Fluorescent Cell Barcoding for Immunophenotyping,implifies implementation of normalization using a bridge control sample..In this chapter, we illustrate the protocol for FCB and recommendations for choosing barcoding dyes and concentrations among other technical considerations.
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Immunophenotyping of Human B Lymphocytes in Blood and in Adipose Tissue,easure markers of activation and/or transcription factors in SAT-derived B cells and B cell subsets. We also show how to evaluate other immune cell types infiltrating the SAT, including T cells, NK cells, monocyte/macrophages, in order to measure relative proportions of these cell types as compared to the blood.
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