Titlebook: Imaging Gene Expression; Methods and Protocol Yaron Shav-Tal Book 2019Latest edition Springer Science+Business Media, LLC, part of Springer

intuition

Monitoring Transcription Factor Oligomerization in Single Living Cells by Number and Brightness Analfluorescence microscopy technique called Number and Brightness analysis (N&B). In this chapter we describe how to acquire and analyze confocal microscopy time-series to provide information about transcription factor oligomerization in living cells using this technique.

形容词

Visualizing Transcription Factor Binding on Mitotic Chromosomes Using Single-Molecule Live-Cell Imaggoing cell division. The few TFs that were observed to remain bound to chromosomes have been termed mitotic bookmarkers and were predicted to play important roles in reestablishing transcription after mitosis. Using live-cell imaging of endogenous TFs in mouse embryonic stem cells, we discovered tha

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Incompetent

erapy, and chemotherapy leading to an overall survival (OS) of 70–80%, but survivors suffer from severe side effects. Based on gene expression, MB is divided in four different molecular subgroups—WNT, SHH, Group 3 (G3), and Group 4 (G4)—which differ in terms of clinics, prognosis, genetic alteration

paradigm

the extensive invasion and diffusion of tumor cells anywhere within the host brain tissue, which makes almost impossible the complete surgical removal. Furthermore, although the blood-brain barrier (BBB) is typically disrupted at the core of GBM, it can be structurally conserved at the tumor periphe

Bureaucracy

Maria Vera,Evelina Tutucci,Robert H. Singerthe extensive invasion and diffusion of tumor cells anywhere within the host brain tissue, which makes almost impossible the complete surgical removal. Furthermore, although the blood-brain barrier (BBB) is typically disrupted at the core of GBM, it can be structurally conserved at the tumor periphe

饥荒

Yachen Ying,Shiqi Mao,Christopher J. Krueger,Antony K. Chenlected through death certificates. Comparison between mortality rates (number of deaths/100000 inhabitants per year) of various countries in the periods 1951-1958 [1118] and 1967-1973 [124, 125] has shown a persistent geographic variability and a clear tendency to increase with time. The annual age-

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预知

Jae Youn Shim,Byung Hun Lee,Hye Yoon Parkst of the cases were published only after the advent of the Nissl stain for ganglion cells. Here our knowledge has been enriched particularly by Bielschowsky and Henneberg; Herxheimer; Pick and Bielschowsky; Schmincke (1909, 1910, 1914); Courville (1930); Christensen (1937), and above all by Foerste

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