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Titlebook: Imaging Gene Expression; Methods and Protocol Yaron Shav-Tal Book 2019Latest edition Springer Science+Business Media, LLC, part of Springer

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书目名称Imaging Gene Expression
副标题Methods and Protocol
编辑Yaron Shav-Tal
视频video
概述Includes cutting-edge methods and protocols.Provides step-by-step detail essential for reproducible results.Contains key notes and implementation advice from the experts
丛书名称Methods in Molecular Biology
图书封面Titlebook: Imaging Gene Expression; Methods and Protocol Yaron Shav-Tal Book 2019Latest edition Springer Science+Business Media, LLC, part of Springer
描述This volume explores the latest updates on microscopy approaches and techniques used by scientists studying in the field of gene expression imaging. These updates cover the technical design of the experiments and the expected outcomes. The chapters in this book are divided into two parts: Part One looks at the output of a gene, in particular the RNA molecules that are copied from the gene itself; and Part Two focuses on chromosomes, chromatin, and factors that bind DNA. Written in the highly successful .Methods in Molecular Biology. series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.. .Cutting-edge and thorough, .Imaging Gene Expression: Methods and Protocols, Second Edition. is a valuable resource for any researcher interested in learning more about this evolving and important field. .
出版日期Book 2019Latest edition
关键词FISH; Drosophila; mRNAs; Endoplasmic Reticulum; ; RNA Pol II; CRISPR; gene expression; Biological Microscopy
版次2
doihttps://doi.org/10.1007/978-1-4939-9674-2
isbn_softcover978-1-4939-9676-6
isbn_ebook978-1-4939-9674-2Series ISSN 1064-3745 Series E-ISSN 1940-6029
issn_series 1064-3745
copyrightSpringer Science+Business Media, LLC, part of Springer Nature 2019
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Detection of mRNA Transfer Between Mammalian Cells in Coculture by Single-Molecule Fluorescent In Siresented here will allow the user to investigate mRNA transfer of most transcripts in any co-culture system. In addition, we present modifications that improve nanotube preservation during the smFISH procedure.
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Programmable Chromosome Painting with Oligopaintswith sequential FISH to label entire genomes with a single denaturation step. Here, we outline a protocol and considerations to scale the Oligopaint technology for fluorescent labeling of whole chromosomes.
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Book 2019Latest editionhese updates cover the technical design of the experiments and the expected outcomes. The chapters in this book are divided into two parts: Part One looks at the output of a gene, in particular the RNA molecules that are copied from the gene itself; and Part Two focuses on chromosomes, chromatin, an
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