Titlebook: ICT Education; 49th Annual Conferen George Wells,Monelo Nxozi,Bobby Tait Conference proceedings 2021 Springer Nature Switzerland AG 2021 ar

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Anchal Garg,Jean-Paul Van Bellespecificities. Here, we describe a fast and simple protocol for small-scale packaging of CRISPR/Cas9 components into AAV vectors. To showcase its potential, we employ this method for screening of gRNAs targeting the murine miR-122 locus in Hepa1–6 cells (using AAV serotype 6, AAV6) or the 5′LTR of t

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Lynn Futcher,Kerry-Lynn Thomson,Apelele Mbuqesage. We will also discuss the installation of a dockerized version of CRISPR Library Designer (CLD) that can be deployed locally or in the cloud to support the end-to-end design of sgRNA libraries for more than 50 different organisms. CLD was built upon E-CRISP to further increase the scope of sgRN

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Malie Zeeman,Romeo Botesequences, while the second provides a broad specificity profile based on cleavage of a massive library of potential off-target DNA sequences. Collectively, these assays may be used to evaluate the specificity of different CRISPR/Cas9 systems on any DNA target sequence in a time- and cost-effective m

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