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Titlebook: ICT Education; 49th Annual Conferen George Wells,Monelo Nxozi,Bobby Tait Conference proceedings 2021 Springer Nature Switzerland AG 2021 ar

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Dane Brown,James Connanls, tissues, and whole animals showing great promise for translational applications. With regard to CRISPR/Cas9, a variety of repurposed systems have been developed to achieve gene knock-out, base editing, targeted knock-in, gene activation/repression, epigenetic modulation, and locus-specific label
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James Connan,Dane Brown,Caroline Watkins expertsThis detailed volume guides readers through strategic planning and user-friendly guidelines in order to select the most suitable CRISPR-Cas system and target sites with high activity and specificity. Methods covering CRISPR gRNA design, CRISPR delivery, CRISPR activity quantification (indel
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Anchal Garg,Jean-Paul Van Bellental biological questions and to target and treat genetic diseases. The CRISPR system, originally derived from bacteria and archaea, can be delivered into cells using different techniques, comprising (1) transfection of mRNA or plasmid DNA, (2) electroporation of plasmid DNA or the Cas9 protein in a
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