Titlebook: Hypoxia; Methods and Protocol Daniele M. Gilkes Book 2024 The Editor(s) (if applicable) and The Author(s), under exclusive license to Sprin

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Genetically Encoded Reporters to Monitor Hypoxia xenografted tumors. Thus, the generation of stably transfected transgenic tumor cell lines, the in vitro calibration of the genetically encoded sensors, the surgical procedures for orthotopic xenografting of tumors in mice, and workflows for the respective sample preparation and microscopy are outlined.

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Application of a Specific and Sensitive NQO1 Turn-On Near-Infrared Fluorescence Probe for Live Canceaging) to rapidly image NQO1 activity in hypoxic and non-hypoxic cancer cells and tumors developed in live mouse xenograft models. The specificity of the dye for NQO1 in all three procedures was verified, and the methods should be useful for both in vitro and in vivo studies.

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Mapping the Fate of Hypoxic Cells Using an Irreversible Fluorescent Switches its effect on cancer, normal processes like embryogenesis, or other pathologies such as ischemia, depend on hypoxia-regulated mechanisms. Given the degradable nature of HIF-1/2α in the presence of oxygen, defining the role of hypoxia in modeling biological processes becomes challenging when a cel

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A Luciferase Reporter Assay to Detect Cellular Hypoxia In Vitro processes, including hematopoiesis, angiogenesis, metabolic changes, cell growth, and apoptosis. Hypoxia-inducible factor-1 (HIF-1) binds to hypoxia response elements (HREs) to selectively induce the expression of various genes in response to hypoxia. Therefore, HREs have been used to develop hypox

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Measuring Pericellular Oxygen Tension for In Vitro Cell Culturelled in conventional cell culture, although chambers that control oxygen in the surrounding gas phase are commercially available. In both cases, it is valuable to understand the pericellular oxygen tension (i.e., the oxygen concentration that cells experience) in cultures. Herein we describe a proce