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Titlebook: Hydrocarbon and Lipid Microbiology Protocols; Ultrastructure and I Terry J. McGenity,Kenneth N. Timmis,Balbina Nogale Book 2016 Springer-Ve

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Protocol for Laser Scanning Microscopy of Microorganisms on Hydrocarbons,-photon excitation. In this protocol, we provide guidance on how to examine microorganisms associated with liquid, viscous and solid hydrocarbons. Practical aspects are discussed including the material and sources, microscopy consumables, software programs and time constraints. The details of mounti
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Bacteria-Mineral Colloid Interactions in Biofilms: An Ultrastructural and Microanalytical Approach,or with dissolved metal ions as primordial biofilms. These bacteria-nanoparticle constructs, exemplified by so-called clay hutches, are accessible to ultrastructural and microanalytical electron microscopical analysis. By this, the spatial arrangements and in part the physiological state of the invo
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Studies of the Ecophysiology of Single Cells in Microbial Communities by (Quantitative) Microautoraysiology of probe-defined single cells in mixed microbial communities. The incorporation of radiolabelled substrates can be quantified by automated image analysis (MARQuant). Quantification of MAR signals can answer more specific questions regarding metabolic activity and function of the microbes. H
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A Correlative Light-Electron Microscopy (CLEM) Protocol for the Identification of Bacteria in Animaon marker gene comparative sequence analysis and fluorescence in situ hybridization (FISH). However, analysis of bacteria and host ultrastructure using transmission electron microscopy (TEM) can be equally important to understand the localization of bacteria and the degree of host-symbiont integrati
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