| 书目名称 | Hydrocarbon and Lipid Microbiology Protocols |
| 副标题 | Ultrastructure and I |
| 编辑 | Terry J. McGenity,Kenneth N. Timmis,Balbina Nogale |
| 视频video | |
| 概述 | Offers readily reproducible, step-by-step laboratory methods.Provides helpful tips and tricks to complement the protocols.Includes troubleshooting advice.Features practical laboratory guidelines to pr |
| 丛书名称 | Springer Protocols Handbooks |
| 图书封面 |  |
| 描述 | This Volume presents key microscopy and imaging methods for revealing the structure and ultrastructure of environmental and experimental samples, of microbial communities and cultures, and of individual cells. Method adaptations that specifically address problems concerning the hydrophobic components of samples are highlighted and discussed. The methods described range from electron microscopy and light and fluorescence microscopy, to confocal laser-scanning microscopy, and include experimental set-ups for the analysis of interfacial processes like microbial growth and activities at hydrocarbon:water interfaces, biofilms and microbe:mineral interfaces. Three forms of fluorescence in situ hybridization - CARD-FISH, MAR-FISH and Two-pass TSA-FISH - are described for the ecophysiological analysis of functionally active microbes in samples. The methods presented will enable readers to obtain an ultrastructural picture of, and identify the key functional microbes in, samples under investigation. This in turn will constitute a key framework for the interpretation of information from other experimental approaches, such as physicochemical analyses and genomic investigations..Hydrocarbon an |
| 出版日期 | Book 2016 |
| 关键词 | Confocal microscopy; Electron microscopy; Fluorescence imaging; Fluorescence in situ hybridization; Micr |
| 版次 | 1 |
| doi | https://doi.org/10.1007/978-3-662-49134-8 |
| isbn_softcover | 978-3-662-56983-2 |
| isbn_ebook | 978-3-662-49134-8Series ISSN 1949-2448 Series E-ISSN 1949-2456 |
| issn_series | 1949-2448 |
| copyright | Springer-Verlag Berlin Heidelberg 2016 |
发表于 2025-3-21 17:04:20
