Titlebook: Human Papillomaviruses; Methods and Protocol Clare Davy,John Doorbar Book 2006 Humana Press 2006 DNA.PCR.Southern Blot.Virus.cell lines.gen

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Detection of Papillomavirus Proteins and DNA in Paraffin-Embedded Tissue Sections,muno-detection varies for different proteins and is dependent on antigen availability. Epitope exposure is sometimes necessary, because the antigen may become masked after formalin fixation and paraffin embedding of the infected tissue. Visualization of both nucleic acid and protein targets can be d

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Analysis of p16INK4a and Integrated HPV Genomes as Progression Markers,rvical cancer development, but there is a high percentage of healthy women infected with HPV that never develop a lesion. Only a small percentage of low-grade dysplasias finally grow out to invasive cancer. Several biomarkers can be used to identify lesions at risk for malignant progression. Overexp

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,Differentiation of HPV-Containing Cells Using Organotypic “Raft” Culture or Methylcellulose,of HPV virions, isolated from tissues, to study viral pathogenesis has been complicated due to the low numbers of HPV virions synthesized and inefficient infection of cells in tissue culture. As an alternative approach, genetic methods have been developed to study the papillomavirus life cycle in it