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Titlebook: Human Papillomaviruses; Methods and Protocol Clare Davy,John Doorbar Book 2006 Humana Press 2006 DNA.PCR.Southern Blot.Virus.cell lines.gen

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书目名称Human Papillomaviruses
副标题Methods and Protocol
编辑Clare Davy,John Doorbar
视频video
概述Includes supplementary material:
丛书名称Methods in Molecular Medicine
图书封面Titlebook: Human Papillomaviruses; Methods and Protocol Clare Davy,John Doorbar Book 2006 Humana Press 2006 DNA.PCR.Southern Blot.Virus.cell lines.gen
描述Leading basic researchers and clinical scientists describe in detail a wide variety of established and cutting-edge techniques they have developed to study the lifecycle and biological properties of the human papillomavirus. The authors use these readily reproducible methods, ranging from PCR to propagation of HPV in vitro, to detect and type papillomavirus infections, study the papillomavirus lifecycle, and to produce and functionally analyze papillomavirus proteins. The protocols follow the successful Methods in Molecular Medicine™ series format, each offering step-by-step laboratory instructions, an introduction outlining the principles behind the technique, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding known pitfalls.
出版日期Book 2006
关键词DNA; PCR; Southern Blot; Virus; cell lines; gene expression; genes; infection; infections; infectious; sacchar
版次1
doihttps://doi.org/10.1385/1592599826
isbn_softcover978-1-61737-526-2
isbn_ebook978-1-59259-982-0Series ISSN 1543-1894 Series E-ISSN 1940-6037
issn_series 1543-1894
copyrightHumana Press 2006
The information of publication is updating

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HPV DNA Detection and Typing in Cervical Scrapes,en be detected by alkaline phosphatase conjugated anti-digoxigenin polyclonal antibodies and substrate followed by optical density reading. Subsequently, EIA-positive PCR products can be typed by a reverse line blot genotyping procedure that, using a miniblotter device, enables typing of up to 39 sa
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,Using an Immortalized Cell Line to Study the HPV Life Cycle in Organotypic “Raft” Cultures,mortalized HFK cell line that supports the complete HPV life cycle has further allowed investigators to study wild-type or mutant papillomaviral genomes that do not confer immortalization. In this chapter, we describe the methodologies that permit the study of the HPV life cycle in this HFK cell lin
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ients do not receive the most effective therapy. Finally, various new treatment been developed, including medical treat­ modalities have ment directed at endocrine pathways in the prostatic cells, balloon dilatation, spirals, temporary or permanent stents, C). nd the application of heat in hyperther
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Ethel-Michele de Villiers PhD,Corinna Whitley,Karin Gunstn a modified Foley-type catheter, could effectively heat periurethral tissues to a depth of about 0.5–1.0 cm (Chap. 13). When the applicator was installed intraurethrally and the feedback thermoregulation was set at 46°C, a cylindrical volume approximately 4 cm in length and 0.5 cm outward from the
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Kiyofumi Egawakidney of an experimental animal recognize that its load has been doubled abruptly? What is the signal for renal hypertrophy? Benign hypertrophy of the prostate is quite different from compensatory hypertrophy of the kidney. It is now known that benign prostatic hypertrophy (BPH) is not a hypertrophy but a be978-1-4612-5478-2978-1-4612-5476-8
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Woei Ling Peh PhD,John Doorbar PhDkidney of an experimental animal recognize that its load has been doubled abruptly? What is the signal for renal hypertrophy? Benign hypertrophy of the prostate is quite different from compensatory hypertrophy of the kidney. It is now known that benign prostatic hypertrophy (BPH) is not a hypertrophy but a be978-1-4612-5478-2978-1-4612-5476-8
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