Titlebook: Human Cytomegaloviruses; Methods and Protocol Andrew D. Yurochko,William E. Miller Book 2014 Springer New York 2014 cytomegalovirus.fibrobl

腐蚀

Infusion

Overview of Human Cytomegalovirus Pathogenesis,isease, as viral infection is now considered to be a strong risk factor for the development of various vascular diseases and to be associated with some types of tumor development. Intense research is currently being undertaken to better understand the mechanisms of viral pathogenesis that are briefly discussed in this chapter.

Decongestant

hidebound

cylinder

Methods to Study the Nucleocytoplasmic Transport of Macromolecules with Respect to Their Impact on only used assays to determine the subcellular localization of a protein, its nucleocytoplasmic shuttling activity, its capacity to export unspliced RNA from the nucleus, and its association with RNA in vivo.

DAFT

,Approaches for the Generation of New Anti-cytomegalovirus Agents: Identification of Protein–ProteinSA), which are designed to search for compounds that act by disrupting the interactions between the HCMV DNA polymerase subunits, but in general can be employed to find inhibitors of any protein–protein interaction of interest; the third is a cell-based assay designed to identify inhibitors of the viral immediate-early 2 (IE2) protein activities.

骚动

Use of Diploid Human Fibroblasts as a Model System to Culture, Grow, and Study Human Cytomegalovirumary human fibroblasts, commencing with culturing and infection of cells and continuing through harvest, titration (determining the infectious capacity of a particular virus preparation), and storage of viral stocks for use in downstream experiments.

incubus

aggressor

Hematopoietic Long-Term Culture (hLTC) for Human Cytomegalovirus Latency and Reactivation,culture with a stromal cell support, and an assay to quantitate infectious centers produced prior to and following a reactivation stimulus. Our method offers a unique way to quantitatively assess HCMV latency and reactivation to study the contribution of viral and host genes in latency and reactivation.

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