Titlebook: Hematopoietic Stem Cells; Methods and Protocol Louis M. Pelus,Jonathan Hoggatt Book 2023 Springer Science+Business Media, LLC, part of Spri

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Lentiviral Transduction of Nonhuman Primate Hematopoietic Stem and Progenitor Cellsl animal model used to study hematopoietic stem and progenitor cell (HSPC) biology, with the capability of serving as a step for the translation of the basic research concepts from small animals to humans. Lentiviral vectors are currently the standard gene delivery vehicles for transduction of HSPCs

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Identification of Nonhuman Primate Hematopoietic Stem and Progenitor Cellsl models such as nonhuman primates (NHPs). Similarity in physiology, size, and life expectation as well as cross-reactivity of most reagents and medications allows for the development of treatment strategies with rapid translation to clinical applications. Especially after the adverse events of HSC

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Utilizing CyTOF to Examine Hematopoietic Stem and Progenitor Phenotypee array of different cell-cell interactions and cell signaling events. The overwhelming diversity of the components that can regulate hematopoiesis, especially when factoring in how the cell surface and intracellular protein expression profiles of hematopoietic stem/progenitor cells and niche compon

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Hematopoietic Stem Cell Identification Postirradiationocesses, as well as the development of treatments to prevent hematopoietic damage or enhance recovery after radiation exposure, often require analysis of bone marrow cells early after irradiation. While flow cytometry methods are well characterized for identification and analysis of bone marrow popu

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Intravital Microscopy for Hematopoietic Studiesas stromal cell components. Together they form the BM microenvironment or HSC niche. HSCs critically depend on signaling from these niches to function and survive in the long term. Significant advances in imaging technologies over the past decade have permitted the study of the BM microenvironment i

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Laser Micromachining of Bone as a Tool for Studying Bone Marrow Biologyoietic stem cells (HSCs) that give rise to all blood cells in the body. It is also the site of origin for malignant blood cells such as leukemia and multiple myeloma, as well as a frequent site of metastasis for many solid tumors including prostate and breast cancer. The following chapter describes

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MSC and HSPC Coculture: Mimicking Ex Vivo Bone Marrow Nichen ex vivo culture system that recapitulates the marrow microenvironment is important to understanding the niche’s regulatory role on HSPC function and improving ex vivo HSPC expansion for clinical transplantation. Herein, a procedure for ex vivo expansion of MSCs from human bone marrow cells and the

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Isolation of Thymus Stromal Cells from Human and Murine Tissueon. The thymic stroma secretes the factors that recruit and sustain T cell progenitors, and they also partake in the shaping of a functional and tolerant T cell receptor repertoire. The damage incurred to the thymic stromal compartment by bone marrow conditioning regimens as well as by the natural a

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Experimental Models of Mouse and Human Hematopoietic Stem Cell Transplantationm cells (HSC) from experimental mouse and human donor groups. These groups could include, but are not limited to, genetically altered populations (gene knockout/knockin models), ex vivo manipulated cell populations, or in vivo modulated cell populations. The basic fundamentals of this process involv