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Titlebook: Hematopoietic Stem Cells; Methods and Protocol Louis M. Pelus,Jonathan Hoggatt Book 2023 Springer Science+Business Media, LLC, part of Spri

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书目名称Hematopoietic Stem Cells
副标题Methods and Protocol
编辑Louis M. Pelus,Jonathan Hoggatt
视频videohttp://file.papertrans.cn/426/425572/425572.mp4
概述Includes cutting-edge techniques.Provides step-by-step detail essential for reproducible results.Contains key implementation advice from the experts
丛书名称Methods in Molecular Biology
图书封面Titlebook: Hematopoietic Stem Cells; Methods and Protocol Louis M. Pelus,Jonathan Hoggatt Book 2023 Springer Science+Business Media, LLC, part of Spri
描述This detailed volume collects updates on the technical advances in hematopoietic stem cell research and incorporates new techniques focused on the molecular/genetic, cellular, and whole organism levels. Exploring methods that apply stress to hematopoiesis, the book also contains chapters focused on better understanding the role of hematopoietic niches and their cellular components, as well as in vivo models that test and quantitate stem cell function and are key to further development of therapeutic applications. Written for the highly successful .Methods in Molecular Biology. series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. .Authoritative and up-to-date, .Hematopoietic Stem Cells: Methods and Protocols. serves as a valued addition to laboratories focused on understanding hematopoietic stem cell biology and the therapeutic advances that can be derived from it..
出版日期Book 2023
关键词Experimental Hematology; Hematopoietic niches; Stress application; Hematopoiesis; Cell function; Therapeu
版次1
doihttps://doi.org/10.1007/978-1-0716-2679-5
isbn_softcover978-1-0716-2681-8
isbn_ebook978-1-0716-2679-5Series ISSN 1064-3745 Series E-ISSN 1940-6029
issn_series 1064-3745
copyrightSpringer Science+Business Media, LLC, part of Springer Nature 2023
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Detection of DNA Damage in Hematopoietic Stem Cellsall, the Comet and Halo assays are powerful means of directly measuring DNA damage. We recommend the below methods for detecting damage in hematopoietic stem cells, but the methods can easily be adjusted for measuring damage in any type of single cells in suspension.
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Evaluating Histone Acetylation in Mouse Hematopoietic Stem and Progenitor Cells Using Chromatin Immuarly under stress, offers the potential for therapeutic intervention. We have utilized Chromatin immunoprecipitation (ChIP) in HSPCs to understand epigenetic regulation in response to ionizing radiation. This technique can be applied reliably to rare hematopoietic cells and offers a powerful tool to explore epigenetic regulation in HSPCs.
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Intravital Microscopy for Hematopoietic Studieshether specific niches exist for distinct developing hematopoietic lineages. Here, we describe our protocol and techniques used to visualize transplanted HSCs in the mouse calvarium, using combined confocal and two-photon IVM.
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