Titlebook: Hedgehog Signaling Protocols; Jamila I. Horabin (Associate Professor) Book 2007 Humana Press 2007 development.gene expression.genes.hybrid

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Confocal Analysis of Hedgehog Morphogenetic Gradient Coupled with Fluorescent , Hybridization of HeIn both Drosophila and vertebrates, the morphogenetic gradient of Hh must be tightly regulated for correct patterning. The posttranslational modification of Hh by a cholesterol adduct participates in such regulation. We have shown that cholesterol modification is necessary for the controlled long-ra

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Germline Clone Analysis for Maternally Acting , Hedgehog Components,tant embryo to survive beyond the first stage of development for which the gene product is required. Zygotic mutations that disrupt the Hedgehog signal transduction pathway typically yield a segment polarity ‘lawn of denticles’ cuticle phenotype. However, an embryo homozygous mutant for a gene can a

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Clonal Analysis of Hedgehog Signaling in , Somatic Tissues, In ., mosaic animals have been widely utilized to study cell fate, growth and patterning, and restriction of cell fate. This chapter describes using FLP recombinase to generate mosaic ., discussing the chromosomes and cross scheme, how to induce the clones, how to properly identify the appropriate

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GAL4/UAS Targeted Gene Expression for Studying , Hedgehog Signaling,e yeast GAL4 transcription factor which activates transcription of its target genes by binding to UAS .-regulatory sites. In ., the two components are carried in separate lines allowing for numerous combinatorial possibilities. The driver lines provide tissue-specific GAL4 expression and the respond

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,Using Immunoprecipitation to Study Protein–Protein Interactions in the Hedgehog-Signaling Pathway,mplexes plays a critical role in the organization and regulation of Hh signaling. Immunoprecipitation (IP) is a powerful tool to study protein-protein interactions and has provided important insights into the regulation of Hh signal transduction. Here, we show how to use IP to study protein-protein

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Darren P. Baker,Frederick R. Taylor,R. Blake Pepinskyigure not only in historical archaeology but also in anthropological archaeology. His work (both past and present) has put him in the forefront of monumental changes in American archaeology in the last 40 years. His personal perseverance in field archaeology has also been an inspiration to new and b