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Titlebook: Hedgehog Signaling Protocols; Jamila I. Horabin (Associate Professor) Book 2007 Humana Press 2007 development.gene expression.genes.hybrid

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发表于 2025-3-21 18:39:11 | 显示全部楼层 |阅读模式
书目名称Hedgehog Signaling Protocols
编辑Jamila I. Horabin (Associate Professor)
视频video
概述Offers various detailed chapters on experimental description, including anecdotal pointers and notes.Intended for Molecular Biologists, Biochemists, and Geneticists
丛书名称Methods in Molecular Biology
图书封面Titlebook: Hedgehog Signaling Protocols;  Jamila I. Horabin (Associate Professor) Book 2007 Humana Press 2007 development.gene expression.genes.hybrid
描述.In both vertebrates and invertebrates, Hedgehog signaling regulates the development of the tissues and structures of the body. In addition, many cancers and diseases arise as a result of misregulation of the pathway, which makes it an incredibly important system to understand. This volume examines various methods used in the analysis of the Hedgehog signaling pathway, bringing together different lines of analyses into one accessible and comprehensive text. This volume provides a single source for investigators, presenting several different experimental approaches, which are all varied and broad in nature, as well as procedures for a variety of different model systems. Researchers will find this volume to be an invaluble tool as they work towards deeper understanding of the underlying molecular events that the Hedgehog signaling pathway regulates. .
出版日期Book 2007
关键词development; gene expression; genes; hybridization; tissue
版次1
doihttps://doi.org/10.1007/978-1-59745-516-9
isbn_softcover978-1-61737-721-1
isbn_ebook978-1-59745-516-9Series ISSN 1064-3745 Series E-ISSN 1940-6029
issn_series 1064-3745
copyrightHumana Press 2007
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Purifying the Hedgehog Protein and its Variants,n cell sources reveals diverse post-translational modifications that affect the potency of the purified protein. Modifications to the N-terminal cysteine with fatty acyl groups results in significant increases in potency, up to 100-fold, when compared with the unmodified protein. Proteolytic clippin
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Application of Sonic Hedgehog to the Developing Chick Limb,egion at the posterior margin of the limb bud and application of Shh-expressing cells or Shh protein to early limb buds mimics polarizing region signaling. The polarizing region (or zone of polarizing activity) is involved in one of the best known cell-cell interactions in vertebrate embryos and is
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Genetic Analysis of the Vertebrate Hedgehog-Signaling Pathway Using Muscle Cell Fate Specification gation of the genetic and cellular basis of vertebrate development. With regard to the Hedgehog (Hh) pathway, forward as well as reverse genetic approaches in this organism have not only validated the roles of evolutionarily conserved players of the signaling cascade, but have also contributed to th
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Cell Surface Marker and Cell Cycle Analysis, Hedgehog Signaling, and Flow Cytometry,ignaling cascade is a master regulator of cell fate during differentiation and is implicated in the development of some neoplasias. Hh signaling affects the expression of cell surface markers of differentiation.We have used the flow cytometer to evaluate the effect of blockage of the Hh signal on th
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