Titlebook: Handbook of ELISPOT; Methods and Protocol Alexander E. Kalyuzhny Book 20051st edition Humana Press 2005 Antigen.Termination.antibody.biolog

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Interferon-γ ELISPOT Assay for the Quantitative Measurement of Antigen-Specific Murine CD8+ T-Cellses. The enzyme-linked immunospot (ELISPOT) is a sensitive technique for the detection of cytokine-producing cells at the single cell level. This assay is rapid and reproducible and permits the direct enumeration of low-frequency antigen-specific T-cells. This protocol describes in detail an interfer

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Epitope Mapping in Multiple Sclerosis Using the ELISPOT Assayutoaggressive response causes the breakdown of the myelin sheaths that protect nerve axons, leading to impaired nerve conduction and subsequent neurodegeneration that are characteristic of MS. Many studies have attempted to determine the exact target within the brain. However, there appear to be mul

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ELISPOT Determination of Interferon-γ T-Cell Frequencies in Patients With Autoimmune Sensorineural H is unclear, it is widely believed that antibody and/or T-cell responses directed against inner ear-specific proteins may mediate ASNHL. Using the enzyme-linked immunospot (ELISPOT) assay, we have recently found that many patients with ASNHL have increased frequencies of peripheral blood T-cells cap

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https://doi.org/10.1007/978-981-13-0308-1 The membranes in these plates are made from either nitrocellulose or polyvinylidene fluoride. These membranes are well suited for ELISPOT because they have high antibody binding capacities and because their white color provides an excellent backdrop for ELISPOT enumeration. These two membranes and,