| 书目名称 | Handbook of ELISPOT | | 副标题 | Methods and Protocol | | 编辑 | Alexander E. Kalyuzhny | | 视频video | | | 概述 | Includes supplementary material: | | 丛书名称 | Methods in Molecular Biology | | 图书封面 |  | | 描述 | In this first book dedicated entirely to the ELISPOT, a critical enzyme-linked immunospot assay used widely in biomedical research, recognized experts with first-hand experience detail how to design, perform, and analyze these assays. The readily reproducible techniques they provide cover a wide variety of topics, including the use of membrane-backed plates, the standardization and validation procedures, the removal of cells from ELISPOT plates, cell separation techniques, and the quantification of ELISPOT data. There are also numerous ELISPOT applications involving animal models, human cells, measles, multiple sclerosis, immune responses, multicytokine detection systems, and immunocytochemistry. Highlights include dual-color and multiplex ELISPOT assays, use of the ELISPOT assay on feline lymphocytes, standardization of the ELISPOT procedure, and combining the ELISPOT assay with immunohistochemistry. | | 出版日期 | Book 20051st edition | | 关键词 | Antigen; Termination; antibody; biology; cell; chemistry; cytokine; cytokines; development; immune response; i | | 版次 | 1 | | doi | https://doi.org/10.1385/1592599036 | | isbn_softcover | 978-1-61737-588-0 | | isbn_ebook | 978-1-59259-903-5Series ISSN 1064-3745 Series E-ISSN 1940-6029 | | issn_series | 1064-3745 | | copyright | Humana Press 2005 |
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发表于 2025-3-21 16:41:37
