Titlebook: Handbook of ELISPOT; Methods and Protocol Alexander E. Kalyuzhny Book 2018Latest edition Springer Science+Business Media, LLC, part of Spri

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Detection of Cross-Reactive B Cells Using the FluoroSpot Assay,s provides a tool for a single-cell analysis of B cell cross-reactivity, for example, with variable and polymorphic antigens found in various pathogens; or analysis of other types of immune responses where analysis of cross-reactivity is of interest. It is also possible to simultaneously analyze B c

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,Multiplex ImmunoSpot® Assays for the Study of Functional B Cell Subpopulations,ction and the secretion of other secretory B cell products, it would then be possible to identify B cell subpopulations that co-express effector molecules. Here we introduce multiplex ELISPOT assays in which measurements of antibody secretion are combined with the detection of Granzyme B, IL-6, IL-1

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,Detecting all Immunoglobulin Classes and Subclasses in a Multiplex 7 Color ImmunoSpot® Assay,ntigen-specific B cells producing IgM, IgA, IgE, IgG., IgG., IgG., and IgG.. We report here the development of a multiplex seven color B cell ImmunoSpot. assay in which the number of antigen-specific B cells can be established simultaneously for all major antibody classes and subclasses, requiring t

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,Multi-Color FLUOROSPOT Counting Using ImmunoSpot® Fluoro-X™ Suite,rated quality control module, with optional GLP and CFR Part 11 compliant package and role-based security, enables data validation, review, and approval with complete audit trails. The extensive multi-format data output and presentation capabilities of the Fluoro-X™ software allow further analysis o

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B-Cell ELISpot Assay to Quantify Antigen-Specific Antibody-Secreting Cells in Human Peripheral Bloossay variability amongst samples from different sources. Here we introduce methods to isolate human peripheral blood mononuclear cells (PBMCs) for quantification of the antigen-specific antibody-secreting cells using the ELISpot assay.

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Cultured ELISpot Assay to Investigate Dengue Virus Specific T-Cell Responses,e Japanese encephalitis virus nor other flaviviruses. Therefore, this assay is likely to be useful in determining the past infecting DENV serotype in immune-epidemiological studies and in dengue vaccine trials.

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