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Titlebook: Handbook of ELISPOT; Methods and Protocol Alexander E. Kalyuzhny Book 2018Latest edition Springer Science+Business Media, LLC, part of Spri

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发表于 2025-3-21 18:31:16 | 显示全部楼层 |阅读模式
书目名称Handbook of ELISPOT
副标题Methods and Protocol
编辑Alexander E. Kalyuzhny
视频video
概述Includes cutting-edge methods and protocols.Provides step-by-step detail essential for reproducible results.Contains key notes and implementation advice from the experts
丛书名称Methods in Molecular Biology
图书封面Titlebook: Handbook of ELISPOT; Methods and Protocol Alexander E. Kalyuzhny Book 2018Latest edition Springer Science+Business Media, LLC, part of Spri
描述.This third edition volume expands on the previous editions with new protocols and up-to-date techniques to help researchers excel in this field. The chapters in this book cover topics such as ELISpot’s sibling (FluoroSpot); ELISpot for influenza, Dengue virus, and tuberculosis; and vaccine research, essentials control, and assay evaluation in ELISpot. Written in the highly successful .Methods in Molecular Biology .series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls..Cutting-edge and comprehensive, .Handbook of ELISPOT: Methods and Protocols, Third Edition. is a valuable resource for both novice researchers and experienced ELISpot users.”.
出版日期Book 2018Latest edition
关键词programmed cell death; stem cell; lime disease; cancer vaccines; tuberculosis
版次3
doihttps://doi.org/10.1007/978-1-4939-8567-8
isbn_softcover978-1-4939-9328-4
isbn_ebook978-1-4939-8567-8Series ISSN 1064-3745 Series E-ISSN 1940-6029
issn_series 1064-3745
copyrightSpringer Science+Business Media, LLC, part of Springer Nature 2018
The information of publication is updating

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发表于 2025-3-21 21:32:20 | 显示全部楼层
Mastering the Computational Challenges of Elispot Plate Evaluation, addressing the analysis step, the plate evaluation. Comparing that fact with the vast amount of analysis advise available for other single cell immune assay, for example, intracellular cytokine staining, the overall impression may be that Elispot evaluation is just simple enough to not require exte
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Automatic Search of Spots and Color Classification in ELISPOT Assay,their morphological variations make it difficult developing a reliable software application. An image recognition method allowing the automatic detection and classification of round objects (spots) on ELISPOT images independently of the registration conditions was developed. The emphasis is done on
发表于 2025-3-22 10:43:48 | 显示全部楼层
,Four Color ImmunoSpot® Assays for Identification of Effector T-Cell Lineages,FN-γ in response to antigen exposure, these assays permit the measurement of the frequency of antigen-specific T cells among white blood cells. These assays therefore are well suited to assess clonal expansions, that is, whether a (Th1) T cell response has been induced to an antigen in a test subjec
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Detection of Cross-Reactive B Cells Using the FluoroSpot Assay,ingle antigen. By reversing the B cell ELISpot and using anti-IgG capture antibodies instead of coated antigen, the specificity of antibodies secreted by B cells can be defined using soluble tagged antigen for detection. When combining this approach with fluorescent detection of the antigen in a B c
发表于 2025-3-22 20:35:13 | 显示全部楼层
,Multiplex ImmunoSpot® Assays for the Study of Functional B Cell Subpopulations,of effector molecules such as cytokines, chemokines, and granzyme. B cell subpopulations releasing such effector molecules have been implicated in immunobiology and a number of diseases..Unlike antigen-specific T cells that can be identified by multimer staining, and then counter-stained to define T
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Multiplexing T- and B-Cell FLUOROSPOT Assays: Experimental Validation of the Multi-Color ImmunoSpotUnique single cell resolution along with high throughput potential sets ELISPOT apart from flow cytometry, ELISA, microarray- and bead-based multiplex assays. The necessity to unambiguously identify individual T and B cells that do, or do not co-express certain analytes, including polyfunctional cyt
发表于 2025-3-23 05:38:58 | 显示全部楼层
,Multi-Color FLUOROSPOT Counting Using ImmunoSpot® Fluoro-X™ Suite, have recently become a major new avenue of development of ELISPOT technology. Advances in assay techniques and the availability of commercial test kits stimulated development of multi-color FLUOROSPOT data analysis platforms. The ImmunoSpot. Fluoro-X™ Software Suite was developed by CTL as an integ
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