Titlebook: Handbook of ELISPOT; Methods and Protocol Alexander E. Kalyuzhny Book 2018Latest edition Springer Science+Business Media, LLC, part of Spri

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B-Cell ELISpot Assay to Quantify Antigen-Specific Antibody-Secreting Cells in Human Peripheral Bloomunological research as compared to the surgically excised and biopsied lymphoid specimens. To determine the functional status of immune system from peripheral blood, the enzyme-linked immunospot (ELISpot) assay is a popular method of choice owing to its high sensitivity, great accuracy, and easy pe

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Cultured ELISpot Assay to Investigate Dengue Virus Specific T-Cell Responses,The use of an in vitro expansion step before the ELISpot distinguishes such “cultured” ELISpots from “ex vivo” ELISpots. Cultured ELISpots have the advantage that lower frequency responses can be analyzed compared to ex vivo ELISpots, but do carry the associated potential distortions of the expansio

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Ex Vivo ELISpot Assay to Investigate iNKT Cell Responses in Acute Dengue Infection,ulation with flow cytometry in functionality studies is challenging. iNKT cells are known to produce both Th1 and Th2 cytokines immediately upon antigen stimulation. Therefore, we have used an ELISpot assay to determine the production of IFN-ɣ and IL-4 after stimulation with KRN7000, which has shown

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AMITY

Utilization of Feline ELISpot to Evaluate the Immunogenicity of a T Cell-Based FIV MAP Vaccine, as heterologous FIV strains from the vaccine subtypes with closely related envelope (Env) sequences. Such protection was mediated by the FIV neutralizing antibodies (NAbs) induced by the vaccines. Remarkably, both prototype and commercial FIV vaccines also conferred protection against heterologous

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