Titlebook: Handbook of ELISPOT; Methods and Protocol Alexander E. Kalyuzhny Book 2018Latest edition Springer Science+Business Media, LLC, part of Spri

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Domenica Di Costanzo,Mariano Mazzaport was published about the subjectivity and operator-dependency of plate evaluation even when using automated reader systems (Janetzki et al., J Immunol Methods 291, 175–183, 2004). Later, the plate evaluation was identified as a main factor causing variability in Elispot results, triggering the i

persistence

Stephen R. Rotman,John R. Saltzman cells, stem-cell like CD8 memory cells, dysfunctional- and senescent CD8 cells all differ in the extent they produce these molecules upon antigen re-encounter. We therefore have developed, and introduce here, a four color T cell ELISPOT assay in which the co-expression levels of IFN-γ, IL-2, TNF-α,

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Raquel E. Aldana,Josephine M. Morenotecting individual cells that secrete multiple analytes vs. random overlays of spots. Here, we present a theoretical and experimental validation study for three and four color T- and B-cell FLUOROSPOT data analysis. The ImmunoSpot. Fluoro-X™ analysis system we used includes an automatic image acquis

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Mental Health Aspects of Voluntary Migrationrated quality control module, with optional GLP and CFR Part 11 compliant package and role-based security, enables data validation, review, and approval with complete audit trails. The extensive multi-format data output and presentation capabilities of the Fluoro-X™ software allow further analysis o

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https://doi.org/10.1007/978-1-4684-3794-2ssay variability amongst samples from different sources. Here we introduce methods to isolate human peripheral blood mononuclear cells (PBMCs) for quantification of the antigen-specific antibody-secreting cells using the ELISpot assay.