Titlebook: Handbook of Biological Confocal Microscopy; James B. Pawley Book 19952nd edition Springer-Verlag US 1995 Confocal.Microscopy.Pawley.biolog

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Thomas Reid’s Lectures on the Fine Artshe new microscope, one can slice incredibly clean thin optical sections out of thick fluorescent specimens; view specimens in planes tilted to, and even running parallel to, the line of sight; penetrate deep light-scattering tissues; gain impressive three-dimensional (3D) views at very high resoluti

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https://doi.org/10.1057/9780230005808y the use of confocal sampling techniques. This chapter will discuss the fundamental considerations that limit the performance of all confocal microscopes. Though at present no commercially available equipment approaches these limits, some simple tests will be described to help the user assess how c

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https://doi.org/10.1007/978-3-476-98873-7 minimal loss of image-forming signal. Significant enhancement of both axial and lateral imaging resolution is also available but only with substantial signal loss. Because of these optical advantages, the confocal laser scanning microscope (CLSM) can clearly image thin optical sections from within

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Concise Key to the Major Groupings,teristics of lasers in relation to their use as an illumination source. Lasers have a number of unique properties compared to other sources emitting electromagnetic radiation, such as arc lamps, which make them an almost ideal light source for use in confocal microscopy. These properties are:

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Sivasubramanian Srinivasan,Ashish Chawlacontrast at which this detail is presented, the depth through the object from which useful information can be derived and the diameter of the useful field are all limited by the performance of the objective. All other imaging components, such as relay optics, Telan systems, tube lenses and eyepieces

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Richard J. Sanders,Dean M. Donahue geometrical optics. Therefore, the author does not present unnecessary mathematics. The simple calculations that are part of the text should be sufficient to understand which calculations are relevant for the design of confocal microscopes.

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