Titlebook: Handbook of Biological Confocal Microscopy; James B. Pawley Book 19952nd edition Springer-Verlag US 1995 Confocal.Microscopy.Pawley.biolog

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Visualization Systems for Multidimensional CLSM Images,e are available from confocal microscope manufacturers, third-party vendors, and other microscopists. The author has attempted to collate important techniques used in these programs. Sources and approximate cost of example systems are given in Table 1.

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Fluorophores for Confocal Microscopy, a century ago (in the case of fluoresceins or rhodamines) or several billion years ago (in the case of phycobiliproteins). Moreover, whereas competition between commercial makers of confocal microscopes stimulates ardent efforts to refine the instrumentation, relatively few companies or academic sc

heterogeneous

General Aspects of Thoracic Anesthesiaeld. We may also produce images in which object height is coded as brightness, or combine the whole data set to provide an isometric view of the object (Fig. 1). It is also possible to use false color to label features of interest or, by simple processing, to obtain stereoscopic pairs (van der Voort

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Book 19952nd editionf, digital aspects of data acquisition and properties of fluorescent dyes, the techniques of 3D specimen preparation and the fundamental limitations, and practical complexities of quantitative confocal fluorescence imaging. Coverage includes practical multiphoton, photodamage and phototoxicity, 3D F

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Francis C. Wells,Aman S. Coonarpletely developed. Computational image processing provides a powerful approach for reducing the systematic errors present in any 3D data set and enhancing the clarity and contrast of relevant features.

Obituary

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Gabriel C. Tender MD,David G. Kline MDeral other chapters in this volume present additional specific information concerning some of these optical components (e.g., light sources, intermediate optics, objective lenses). Our purpose is to treat the illumination path as an integral system and describe its contribution to the overall performance of the confocal microscope.