Titlebook: HIV Protocols ; Vinayaka R. Prasad,Ganjam V. Kalpana Book 2024Latest edition The Editor(s) (if applicable) and The Author(s), under exclus

的阐明

Book 2024Latest editionw modifications to the viral RNA that impacts HIV biology, and new types of intracellular compartments. The chapters in this book are organized into seven parts and cover topics such as HIV latency reactivation via single molecule RNA detection; T cell responses; new and efficacious anti-HIV CAR T c

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Correlative Imaging to Detect Rare HIV Reservoirs and Associated Damage in Tissues imaging (MSI) and laser capture microdissection, could further expand spatial imaging capabilities into high-resolution OMIC approaches such as proteomic, lipidomics, small molecule, and drug discovery. Here, we will describe a protocol to integrate the detection of rare viral reservoirs with imaging mass spectrometry.

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https://doi.org/10.1057/9780230510456racterization of the assembly of viral proteins at the plasma membrane of infected host cells at the single protein level. Here, we describe the TIRF equipment, the T-cell culture for HIV-1, the sample preparation for single-molecule localization microscopies such as PALM and STORM, acquisition protocols, and Gag assembling cluster analysis.

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https://doi.org/10.1007/978-3-030-13016-9. Using this technique, nuclear import of infectious particles can be monitored in both primary and cell culture models. In response to host factor depletion or restriction factors, changes in HIV-1 nuclear import can be effectively measured using the nuclear import kinetics (NIK) assay.

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https://doi.org/10.1057/9780230281530A from HIV-1-infected primary CD4. T-cells based on immunoprecipitation. The enriched RNA with m.A modifications can be used in a variety of downstream applications to determine the methylation status of viral or cellular RNA at resolution from transcript level down to single nucleotide.

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Visualizing HIV-1 Assembly at the T-Cell Plasma Membrane Using Single-Molecule Localization Microscoracterization of the assembly of viral proteins at the plasma membrane of infected host cells at the single protein level. Here, we describe the TIRF equipment, the T-cell culture for HIV-1, the sample preparation for single-molecule localization microscopies such as PALM and STORM, acquisition protocols, and Gag assembling cluster analysis.

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Method for the Enrichment of ,-Methyladenosine-Modified Cellular and HIV-1 RNAA from HIV-1-infected primary CD4. T-cells based on immunoprecipitation. The enriched RNA with m.A modifications can be used in a variety of downstream applications to determine the methylation status of viral or cellular RNA at resolution from transcript level down to single nucleotide.

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