Titlebook: Golgi; Methods and Protocol Yanzhuang Wang,Vladimir V Lupashin,Todd R. Graham Book 2023 The Editor(s) (if applicable) and The Author(s), un

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Algorithm for Modern Electron Microscopic Examination of the Golgi Complexsident proteins is not trivial. Fast development of microscopic methods generates a huge difficulty for Golgi researchers to select the best protocol to use. Modern methods of light microscopy, such as super-resolution light microscopy (SRLM) and electron microscopy (EM), open new possibilities in a

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High-Pressure Freezing Followed by Freeze Substitution: An Optimal Electron Microscope Technique to t technique that freezes the samples at high pressure (~2100 bar) and low temperature (−196 °C) within milliseconds. This ultrarapid fixation enables simultaneous immobilization of all cellular components and preserves the samples in a near-native state. This facilitates the study of dynamic process

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Modeling the Cryo-EM Structure of the Exocyst Complexes from the donor compartments to the membrane of receptor compartments. The exocyst complex is an octameric MTC that tethers the post-Golgi secretory vesicles to the plasma membrane. To learn the function and regulation of the exocyst complex, it is crucial to understand the structure of the comple

AUGUR

Quantitatively Assessing Co-Localization of Golgi Proteins by Distance Analysis Using the DiAna Softcalization assays and is based on measuring distances between pairs of objects representative of the two proteins. It makes use of a relatively recently developed ImageJ plugin called DiAna, which employs semi-automated object recognition and subsequent distance analysis of the recognized objects. T

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E. Beckmann,I. Broser,R. Broserociated constituents. Here, we describe an antibody staining method to detect Golgi-associated proteins in . larval salivary glands, using the .-Golgi protein Lava lamp and the clathrin adaptor AP-1 as a suitable example. Golgi bodies immunostained using this protocol can be visualized using confoca

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Luminescent Processes in Semiconductorscellulosic cell wall. The Golgi apparatus houses numerous cell wall-synthesizing or cell wall-modifying enzymes to generate the complex cell wall structure. However, several putative cell wall biosynthetic candidates await characterization, which requires verification of the subcellular localization