Titlebook: Golgi; Methods and Protocol Yanzhuang Wang,Vladimir V Lupashin,Todd R. Graham Book 2023 The Editor(s) (if applicable) and The Author(s), un

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Crater-Hopping: Observing the Moon on Day 6,ed, and then structures are labelled using primary antibodies directly conjugated to fluorophores, or, more commonly, first with an antibody against an antigen of interest followed by a secondary antibody conjugated to a fluorophore that binds to the primary antibody. Fluorescence can be visualized

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https://doi.org/10.1007/978-88-470-2637-7or simultaneous imaging. Using this technology, we are now able to observe the fine details of various dynamic events going on in living cells, such as membrane traffic and organelle dynamics. The retention using selective hooks (RUSH) system is a powerful tool to control synchronous release of natu

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https://doi.org/10.1007/978-1-84628-154-9sident proteins is not trivial. Fast development of microscopic methods generates a huge difficulty for Golgi researchers to select the best protocol to use. Modern methods of light microscopy, such as super-resolution light microscopy (SRLM) and electron microscopy (EM), open new possibilities in a

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