Titlebook: Golgi; Methods and Protocol Yanzhuang Wang,Vladimir V Lupashin,Todd R. Graham Book 2023 The Editor(s) (if applicable) and The Author(s), un

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Quantification of Golgi Protein Mislocalization to the Budding Yeast Vacuolend retrograde vesicular trafficking. Disruptions in these signals or in the retrograde pathways often lead to mislocalization of Golgi proteins to the vacuole in budding yeast. The extent of vacuolar mislocalization can be quantified through colocalization of GFP-tagged Golgi proteins with fluoresce

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Microscopy and Immunocytochemistry-Based Methods to Study Cell Wall Biosynthetic Enzymes in the Golgcellulosic cell wall. The Golgi apparatus houses numerous cell wall-synthesizing or cell wall-modifying enzymes to generate the complex cell wall structure. However, several putative cell wall biosynthetic candidates await characterization, which requires verification of the subcellular localization

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Studying the Organization of the Golgi by Super-Resolution Microscopyibution of Golgi proteins to understand Golgi functions. The .-to-. or axial localization of a Golgi protein can be obtained using our previously developed method, Golgi protein localization by imaging centers of mass (GLIM), in nocodazole-induced Golgi ministacks (hereafter referred to as ministack

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Studying Golgi Structure and Function by Thin Section TEMM) and for 3,3′-diaminobenzidine (DAB) cytochemical staining and pre-embedding immunolabelling to localize specific Golgi proteins. Furthermore, we demonstrate how the Golgi morphology can be elucidated by classifying the Golgi membranes using Microscopy Image Browser—a software that provides anonym