Titlebook: Glycovirology; Methods and Protocol Yasuo Suzuki Book 2022 The Editor(s) (if applicable) and The Author(s), under exclusive license to Spri

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Receptor-Binding Assay for Avian Influenza Viruses,itiate their invasion to host cells. Unlike well-known theories in human and avian influenza viruses, barriers laying between interspecies transmission of influenza viruses among bird species are not well understood. Recently, it was speculated that glycan binding of the HA to fucosylated Siaα2–3Gal

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Characterization of Influenza Virus Binding to Receptors on Isolated Cell Membranes,and mammals determine viral host range, tissue tropism, and pathogenicity. Here, we describe method that allows to characterize binding of IVs to biologically relevant cellular receptors using a conventional solid-phase enzyme-linked assay. In this method, we isolate plasma membranes from respirator

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CLEAR

Solid-Phase Binding Assay for Ganglioside Binding of Human Respiroviruses,ter by utilizing glycoproteins or glycosphingolipids (gangliosides) containing sialic acid on the cell membrane. We developed a solid-phase binding assay to evaluate hPIV-1, hPIV-3, and Sendai virus’ abilities to bind to different types of gangliosides. hPIV1 and hPIV3 show strong binding to neolact

GLIDE

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Roles of Sialyl Glycans in HCoV-OC43, HCoV-HKU1, MERS-CoV and SARS-CoV-2 Infections,ng pandemic SARS-CoV-2 (COVID-19) with high mutability giving some variants causing severe illness and death have been reported to attach to sialyl receptors via their spike (S) glycoproteins and via additional short spikes, hemagglutinin-esterase (HE) glycoproteins, for HCoV-OC43 and HCoV-HKU1. The

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Assays for Determining the Sialidase Activity of Influenza Viruses and Monitoring Influenza Virus Sviruses. The fluorescent assay is cost-effective and applicable for many laboratories and is, therefore, commonly used for global monitoring of the NA inhibitor susceptibility of influenza viruses. Here, I describe, in detail, protocols for the fluorescence-based NA activity assay and the NA inhibit

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