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Titlebook: Glycovirology; Methods and Protocol Yasuo Suzuki Book 2022 The Editor(s) (if applicable) and The Author(s), under exclusive license to Spri

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Nikolaus Knoepffler,Nikolai Münchar, and tissue levels and can be used to study the glycans of virological samples such as virus and their hosts. It also enables the discrimination of isomers, which is difficult via mass spectrometry. In this paper, we describe in detail the principle and method of the structural analysis of .-type glycans using the proposed method.
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Reservoirs of Visceral Leishmaniasisomain protein was amplified with PCR and cloned into an expression vector. Six His-tagged PA endonuclease domain proteins were expressed in . and purified with Ni.-agarose resin and imidazole using an ion-exchange column. Using the recombinant PA endonuclease domain protein, an endonuclease assay was performed.
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https://doi.org/10.1007/978-3-322-91508-5-binding and esterase activities of HEF in vitro. The glycan-bind property is monitored through glycan microarray, MDCK cell-binding assay, Hemagglutination assay, solid-phase lectin binding assay, and immunofluorescence of tissue sections, and its esterase property is analyzed via esterase enzymatic activity assay.
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Preparation of Recombinant PA Endonuclease Domain Protein of Influenza A Virus and Its Application omain protein was amplified with PCR and cloned into an expression vector. Six His-tagged PA endonuclease domain proteins were expressed in . and purified with Ni.-agarose resin and imidazole using an ion-exchange column. Using the recombinant PA endonuclease domain protein, an endonuclease assay was performed.
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