Titlebook: Glycosaminoglycans; Chemistry and Biolog Kuberan Balagurunathan,Hiroshi Nakato,Umesh R. Des Book 2015 Springer Science+Business Media New Y

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Enzymatic Synthesis of Heparan Sulfate and Heparinnd can be used as a precursor in the enzymatic synthesis of HS-like polysaccharides. Here, we describe an enzymatic approach to synthesize several specifically sulfated HS polysaccharides for biological studies using the heparosan backbone and a combination of recombinant biosynthetic enzymes such as C5-epimerase and sulfotransferases.

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https://doi.org/10.1007/978-3-662-12243-3tion relations is, however, hampered due to the lack of availability of HS in sufficient quantities as well as the molecular heterogeneity of naturally occurring HS. Enzymatic synthesis of HS is an attractive alternative to the use of naturally occurring HS, as it reduces molecular heterogeneity, or

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https://doi.org/10.1007/978-3-642-96252-3 which bind their target proteins to induce conformational changes, compete with other ligands, or facilitate the formation of signaling complexes. One example, the extensively studied heparin pentasaccharide sequence—which binds antithrombin-III, inducing a conformational change that increases its

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https://doi.org/10.1007/978-3-662-00125-7 studies of heparan sulfate are often challenging due to the heterogeneity and complexity of the HS molecules. Radioisotope metabolic labeling of HS in cellular systems has enabled the elucidation of HS structures as well as the interactions between HS and proteins. However, radiolabeled structures