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Titlebook: Glycosaminoglycans; Chemistry and Biolog Kuberan Balagurunathan,Hiroshi Nakato,Umesh R. Des Book 2015 Springer Science+Business Media New Y

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Abweichungen, Pathologie und Irreseinetic apparatus. The protocols for the gel preparation are based on the variations of the acrylamide/bisacrylamide and buffer concentrations. Different approaches for the extraction and purification of the disaccharides of various biologic samples and pharmaceutical preparations are also stressed.
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Preparation of Isotope-Enriched Heparan Sulfate Precursors for Structural Biology Studiesion of stable isotope-enriched HS precursors has facilitated the HS structural analysis by NMR spectroscopy and mass spectrometry. Herein we describe a simple method to prepare isotopically enriched HS precursors.
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Capillary Electrophoretic Analysis of Isolated Sulfated Polysaccharides to Characterize Pharmaceuticulfate saccharides from urine, their digestion by deployment of heparinase enzymes (Ernst et al., Crit Rev Biochem Mol Biol 30:387–444, 1995), resolution of species through use of orthogonal digestions, and analysis of the resulting disaccharides by capillary electrophoresis.
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Die einzelnen Geisteskrankheiten,bile linker. The linker cleavage from the resin is performed in a continuous-flow photoreactor under chemically mild conditions. The described approach will serve as a general scheme to systematically access oligosaccharides of all GAG families.
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Räumliches Wahrnehmen und Vorstellenlic labeling of heparan sulfate with [.S]sulfate or [.H]glucosamine in cell cultures is described, in addition to characterization of polysaccharide chain length and degree of .-sulfation. Methods to measure NDST enzyme activity are also presented.
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Automated Synthesis of Chondroitin Sulfate Oligosaccharidesbile linker. The linker cleavage from the resin is performed in a continuous-flow photoreactor under chemically mild conditions. The described approach will serve as a general scheme to systematically access oligosaccharides of all GAG families.
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