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Titlebook: Glycosaminoglycans; Methods and Protocol Kuberan Balagurunathan,Hiroshi Nakato,Yukio Saijoh Book 2022Latest edition Springer Science+Busine

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Lehrbuch der Physiologie des Menschenort, 1-phenyl-3-methyl-5-pyrazolone (PMP) and deuterated d5-PMP are used for differential disaccharide labeling and profiling of chondroitin sulfate GAG by high performance liquid chromatography (HPLC) coupled with mass spectrometry (MS).
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Lehrbuch der Physiologie des Menschenhapter, metabolic labeling of heparan sulfate with [.S]sulfate or [.H]glucosamine in cell cultures is described, in addition to characterization of polysaccharide chain length and degree of .-sulfation. Methods to measure NDST enzyme activity are also presented.
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https://doi.org/10.1007/978-3-662-43158-0in molecular weight markers or a single broad standard calibrant and used to characterize the molecular weight distribution of polydisperse samples or the peak molecular weight of monodisperse, or approximately monodisperse, heparin fractions. The same technology can be adapted for use with other glycosaminoglycans.
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Fluorous-Tag-Assisted Synthesis of GAG-Like Oligosaccharidesluorous tail allows the quick purification of the desired glycosylation product by performing a simple fluorous solid-phase extraction (F-SPE). The described fluorous-tag–assisted glycosylation strategy greatly facilitates the assembly of building blocks, speeding up the preparation of biologically relevant GAG-like oligomers.
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Quantitative Disaccharide Profiling of Glycosaminoglycans from Two Different Preparations by PMP andort, 1-phenyl-3-methyl-5-pyrazolone (PMP) and deuterated d5-PMP are used for differential disaccharide labeling and profiling of chondroitin sulfate GAG by high performance liquid chromatography (HPLC) coupled with mass spectrometry (MS).
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Chromatographic Molecular Weight Measurements for Heparin , Its Fragments and Fractions, and Other Gin molecular weight markers or a single broad standard calibrant and used to characterize the molecular weight distribution of polydisperse samples or the peak molecular weight of monodisperse, or approximately monodisperse, heparin fractions. The same technology can be adapted for use with other glycosaminoglycans.
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Book 2022Latest edition enzymatic synthesis of GAGs; biophysical, biochemical, and computational analysis of GAG-protein interactions; molecular and genetic approaches to manipulating GAG cell biology; and molecular and genetic methods involving animal models and organoids. This volume also offers insight and guidance to
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