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Titlebook: Glycosaminoglycans; Methods and Protocol Kuberan Balagurunathan,Hiroshi Nakato,Yukio Saijoh Book 2022Latest edition Springer Science+Busine

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书目名称Glycosaminoglycans
副标题Methods and Protocol
编辑Kuberan Balagurunathan,Hiroshi Nakato,Yukio Saijoh
视频video
概述Includes cutting-edge methods and protocols.Provides step-by-step detail essential for reproducible results.Contains key notes and implementation advice from the experts
丛书名称Methods in Molecular Biology
图书封面Titlebook: Glycosaminoglycans; Methods and Protocol Kuberan Balagurunathan,Hiroshi Nakato,Yukio Saijoh Book 2022Latest edition Springer Science+Busine
描述.This second edition volume expands on the previous edition with new information on the structural analysis of glycosaminoglycans (GAGs); chemical and enzymatic synthesis of GAGs; biophysical, biochemical, and computational analysis of GAG-protein interactions; molecular and genetic approaches to manipulating GAG cell biology; and molecular and genetic methods involving animal models and organoids. This volume also offers insight and guidance to non-glycoscience researchers who are performing advanced experiments on GAGs. Written in the highly successful .Methods in Molecular Biology. series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. .Cutting-edge and authoritative, .Glycosaminoglycans: Methods and Protocols, Second Edition. is a valuable experimental manual for practicing researchersincluding graduate and post-doctoral fellows, and provides them with the latest methodologies and practical tips to overcoming barriers in understanding the chemistry and biology of GAGs. .
出版日期Book 2022Latest edition
关键词GAG oligosaccharides; heparin; biosynthetic enzymes; Biomechanical Properties; NMR approaches
版次2
doihttps://doi.org/10.1007/978-1-0716-1398-6
isbn_softcover978-1-0716-1400-6
isbn_ebook978-1-0716-1398-6Series ISSN 1064-3745 Series E-ISSN 1940-6029
issn_series 1064-3745
copyrightSpringer Science+Business Media, LLC, part of Springer Nature 2022
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Aqueous Molecular Dynamics for Understanding Glycosaminoglycan Recognition by Proteinsd interpretation of stability, specificity, and conformational properties of GAGs, while also elucidating their interactions with amino acid residues of a protein at an atomistic level in presence of water.
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Nuclear Magnetic Resonance Methods in Structural Characterization of GlycosaminoglycansMR methods discussed here cover all three isotopes (.H, .C, and .N) that can be exploited in structural analysis of GAGs. These NMR methods are described from an overall standpoint, to be applied to any GAG family, extracted from either natural or synthetic sources and destined to either basic research or pharmaceutical applications.
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Preparation and Characterization of Heparan Sulfate-Derived Oligosaccharides to Investigate Protein–ating, engineering, and characterizing heparan sulfate-derived oligosaccharides and approaches based on high-performance liquid chromatography (HPLC), nuclear magnetic resonance (NMR), and bio-layer interferometry (BLI) to study their structures, modifications, and interactions.
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