Titlebook: Glycobiology Protocols; Inka Brockhausen Book 2007 Humana Press 2007 Lipid.Oligosaccharid.Polysaccharid.biochemistry.enzymes.gene expressi

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Carlos Fernández de Casadevante Romaniat would be of interest as therapeutic compounds. Because of sequence diversity and limited experimental data, molecular modeling of these enzymes is not straight-forward and utilizes the most recent tools, such as fold recognition programs.

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Application of Fluorophore-Assisted Carbohydrate Electrophoresis for the Study of the Dolichol Pyroor a facile, accurate, and sensitive non-radioactive method for LLO pathway analysis based on fluorophore-assisted carbohydrate electrophoresis (FACE). It is feasible to analyze almost any component in the LLO pathway with the application FACE, from sugar precursors to mature LLO (Glc.Man.GlcNAc.-P-P-dolichol).

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Structural Analysis of Naturally Occurring Sialic Acids,of trimethylsilylated methyl ester derivatives, heptafluorobutylated methyl ester derivatives, or pertrimethylsilylated derivatives. The fluorescent Sia derivatives are prepared by reaction with 1,2-diamino-4,5-methylenedioxybenzene. For the identification of the different Sia derivatives, detailed GLC, HPLC, EI/MS, and ESI/MS data are included.

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Assay for a Galactosyltransferase Involved in the Assembly of the O7-Antigen Repeat Unit of ,,al substrate, was synthesized and employed in GT assays using bacterial membranes as the enzyme source. The assay is simple and allows optimization and characterization of the enzyme reaction. Similar protocols can be used to assay other GTs in the O-chain biosynthesis pathways.

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Micromethods for the Characterization of Lipid A-Core and O-Antigen Lipopolysaccharide,hanism of synthesis and assembly that requires the coordinated participation of many genes and gene products. This chapter describes a collection of methods routinely used in our laboratory for the characterization of LPS in . and other bacteria.

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