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Titlebook: Glycobiology Protocols; Inka Brockhausen Book 2007 Humana Press 2007 Lipid.Oligosaccharid.Polysaccharid.biochemistry.enzymes.gene expressi

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书目名称Glycobiology Protocols
编辑Inka Brockhausen
视频video
概述Includes supplementary material:
丛书名称Methods in Molecular Biology
图书封面Titlebook: Glycobiology Protocols;  Inka Brockhausen Book 2007 Humana Press 2007 Lipid.Oligosaccharid.Polysaccharid.biochemistry.enzymes.gene expressi
描述Glycobiology involves studies of complex carbohydrates and posttrans- tional modifications of proteins, and has become an important interdiscip- nary field encompassing chemistry, biochemistry, biology, physiology, and pathology. Although initial research was directed toward elucidation of the different carbohydrate structures and the enzymes synthesizing them, the field has now moved toward identifying the functions of carbohydrates. The pro- cols described in Glycobiology Protocols form a solid basis for investigations of glycan functions in health and disease. The cloning of many of the genes participating in glycosylation processes has helped to enhance our knowledge of how glycosylation is controlled, but has also added another dimension of complexity to the great heterogeneous variety of the structures of the oligos- charides of glycoproteins, proteoglycans, and glycolipids. A family of similar enzyme proteins exists for each glycosylation step. Glycosyltransferases are extremely specific for both the nucleotide sugar donor and the acceptor s- strate, but many other factors control sugar transfer, including the locali- tion and topology of enzymes, cofactors, possible chapero
出版日期Book 2007
关键词Lipid; Oligosaccharid; Polysaccharid; biochemistry; enzymes; gene expression; physiology; proteins
版次1
doihttps://doi.org/10.1385/1597451673
isbn_softcover978-1-61737-638-2
isbn_ebook978-1-59745-167-3Series ISSN 1064-3745 Series E-ISSN 1940-6029
issn_series 1064-3745
copyrightHumana Press 2007
The information of publication is updating

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Nonradioactive ,-Sialidase Screening Assay,an be applied to screen a large number of samples quickly and reliably during enzyme purification, for testing inhibitors, and for monitoring TS activity during the production of monoclonal antibodies (.)..This chapter focuses on the main steps of this assay and gives detailed instructions for perfo
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,β-Galactoside α2,6-Sialyltransferase and the Sialyl α2,6-Galactosyl-Linkage in Tissues and Cell Liny to the isoform-specific regions. Very high levels of ST6Gal.I activity result in high levels of SNA reactivity and are associated with the expression of the H transcript in colon and liver cell lines, and of the X transcript in B cells.
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Analysis of the Glycodynamics of Primary Osteoblasts and Bone Cancer Cells,.H]thymidine incorporation as a measure of cell proliferation, and to flow cytometry of terminal deoxynucleotidyl transferase dUTP-mediated nick end labeling (TUNEL) and annexin V-stained cells as a measure of apoptosis. These in vitro studies are aimed at an understanding of the role of glycosylati
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High-Throughput Quantitation of Metabolically Labeled Anionic Glycoconjugates by Scintillation Prox. The samples are counted using a multi-detector instrument for scintillation proximity assays, such as the Wallac 1450 Microbeta® Trilux, designed for detection of samples in 96-well plates and, as such, can be a high-throughput system. The bound anionic GCs can be visualized by sodium dodecyl sulf
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